1 | | Gene manipulation in the post-genomics era | | 1 |
2 | | Basic techniques | | 15 |
3 | | Cutting and joining DNA molecules | | 36 |
4 | | Basic biology of plasmid and phage vectors | | 55 |
5 | | Cosmids, phasmids, and other advanced vectors | | 75 |
6 | | Gene-cloning strategies | | 96 |
7 | | Sequencing genes and short stretches of DNA | | 126 |
8 | | Changing genes : site-directed mutagenesis and protein engineering | | 141 |
9 | | Bioinformatics | | 157 |
10 | | Cloning in bacteria other than Escherichia coli | | 179 |
11 | | Cloning in Saccharomyces cerevisiae and other fungi | | 202 |
12 | | Gene transfer to animal cells | | 218 |
13 | | Genetic manipulation of animals | | 251 |
14 | | Gene transfer to plants | | 274 |
15 | | Advanced transgenic technology | | 299 |
16 | | The organization and structure of genomes | | 323 |
17 | | Mapping and sequencing genomes | | 346 |
18 | | Comparative genomics | | 373 |
19 | | Large-scale mutagenesis and interference | | 394 |
20 | | Analysis of the transcriptome | | 407 |
21 | | Proteomics I - expression analysis and characterization of proteins | | 425 |
22 | | Proteomics II - analysis of protein structures | | 441 |
23 | | Proteomics III - protein interactions | | 453 |
24 | | Metabolomics and global biochemical networks | | 472 |
25 | | Applications of genomics : understanding the basis of polygenic disorders and identifying quantitative trait loci | | 485 |
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